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|Title||NIBR Developed Cell Cryopreservation and Transplantation Techniques for Restoring the Class I Endangered Fish Species|
NIBR Developed Cell Cryopreservation and Transplantation Techniques for Restoring the Class I Endangered Fish Species
NIBR announced that it has succeeded in cryopreserving germline stem cells of 4 endangered fish and transplanting the germ cells of Miho spine loach (Cobitis choii) into surrogate loaches for proliferation.
Cryopreservation is a method for long term storage of cells or tissues at ultra-low temperature (below -136 °C, i.e.).
Cobitis choii is a Korean endemic species and listed as Class I endangered species. The fish is at risk of extinction due to water pollution and extensive habitat loss. It was found for the first time in 1984, in Miho River, Chungcheongbuk-do. It is 8-10cm length and lives in clean and slow moving waters.
The fish is endemic species and has conservation values across the globe; thereby the restoration efforts are urgently required.
Since 2015, NIBR has conducted the project, end “Development of Applied Technologies Using Fish Germline Stem Cells”. From the research, NIBR successfully developed the stem cell cryopreservation techniques for 4 endangered fish and applied them to proliferation.
They found that the survival rate of the germline stem cells is largely affected by the types and concentrations of cryoprotectants, cooling rates, and thawing temperatures, etc.
Among the cryopreserved cells of the 4 endangered fish, the germ cells of Cobitis choii were transplanted into sterilized loaches. The donor-derived germline stem cells initiated either spermatogenesis or oogenesis in the recipient gonads, depending on the sex of the recipient.
Using the loaches with the germ cells transplanted for artificial insemination resulted in the production of 7,576 offspring late last year. Researchers confirmed that the offspring’s genes match the donors’.
The cryopreservation and transplantation techniques used for Cobitis choii can be an effective alternative to restore and proliferate other threatened and endangered fish.
“We have successfully proliferated Cobitis choii with the new techniques, and it shows the possibility of restoring extinct species if we secure their germ stem cells,” Woonsuk, Baek, president of NIBR said. “We expect the techniques to be utilized for restoring other endangered fish.”
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